Caseinolytic and Fibrinolytic Activities of Human Plasma in Starch Block Electrophoresis

Abstract

Summary

Human plasma was subjected to starch electrophoresis with veronal buffer at pH 8.6, μ = 0.05. The veronal buffer eluates of each of the 40 sections into which the block was cut were used for the study of caseinolytic and fibrinolytic activity after activation with streptokinase or urokinase. A high fibrinolytic activity was induced by streptokinase in the γ-globulin fraction. A high caseinolytic activity was induced by urokinase in the β-globulin fraction and by streptokinase in the α₁-globulin fraction. Caseinolytic activity in the α₁-globulin fraction was not inhibited by a concentration of epsilon-aminocaproic acid (2 × 10^-3 M) which gave a marked inhibition of the fibrinolytic activity elicitable in the β- and γ-globulin fractions. Inhibition of the caseinolytic activity in the α₁-globulin fraction was effected by dicarbobenzoxy-lysine (2 × 10^-3 M) which had only a slight inhibitory effect on the fibrinolytic activity of the β- and γ-globulin fractions.