Skeletal Cell Proliferative Rates

Abstract

Summary

An autoradiographic study of mouse skeletal cell proliferation rates using increasing doses of H³TDR were reported. The data showed that in both the osteogenic cells of the femoral periosteum and epiphyseal plate the labeling indices increased rapidly with increasing doses of H³TDR to 1.0 μc/g body weight and less rapidly with larger doses. It was concluded from this study that previously reported estimates of labeling indices based on lower doses of administered H³TDR are lower than the actual values. A dose of 1.0 μc H³TDR/g body weight is recommended for the estimates of labeling indices using the “flash labeling” technique. However, doses of 1.0 μc and above are not to be used for cell migration or cell kinetic studies.