Abstract
Summary
Conventional methods for introduction of I131 may modify or destroy the serological reactivity of labile microbial antigens. A modified microdiffusion iodination method that eliminates the exposure of antigens to excess oxidizing agent produced satisfactory trace labeling of purified protective antigen of Bacillus anthracis and enterotoxin B of Staphylococcus aureus without detectable change in precipitating activity. The iodinated antigens proved suitable for use in the ammonium sulfate coprecipitation technique of Farr.
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