Abstract
Summary
The kinetics of the hemolytic antibody response in mice to a single injection of sheep erythrocytes were determined by quantitating the number of hemolytic foci which appeared in an agar-erythrocyte over-lay on thin sections of mouse spleen. After immunization, the number of foci rose to a peak and then declined gradually to levels which persisted through 113 days. At 210 days after immunization, foci activity had merged with control levels. On the other hand, the plaque-forming cell method of Jerne showed an increase in the number of plaque-forming cells until a peak was reached with a subsequent rapid decline to control levels 70-80 days after immunization. It is concluded that the frozen section technique is a sensitive method for the discrimination and enumeration of cells containing specific antibody in tissues.
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