Abstract
Discussion and summary
Rubella virus produced distinct plaques with sharp boundaries in monolayer cultures of SIRC cells under a double agar overlay. Rubella virus plaques were also obtained in Vero, MA-111, and RK-13 cell cultures by using the same overlay, but the plaques were not clear and boundaries were not as distinct as those found in SIRC monolayers. A linear relationship was observed between plaque counts and virus concentration, permitting precise quantitative virus assays. Plaque reduction neutralization tests for rubella virus gave reproducible titers, and proved to be more sensitive than those done by the CPE method using monolayers in tubes with fluid medium. The virus dose employed also appeared to be less critical.
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