Abstract
Summary
Adenosine triphosphate (ATP) content of spermatozoa, semen and seminal plasma was obtained using a modification of the firefly bioluminescence assay method. Washed sperm of 6 mammalian species showed an ATP content ranging from 1.3 to 10.1 while cock sperm contain less than 1 μg ATP per 108 sperm. Separate assay of whole semen and seminal plasma indicated that the plasma and sperm contents add up approximately to the semen content with indication of some loss from bull and dog seminal plasma.
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