Abstract
Summary
The insulin-like activity found in urine protein from obese subjects during fasting was characterized by benzoic acid precipitate and Sephadex column chromatography. The major portion of the urine insulin-like activity was found in a single protein peak which was homogeneous by polyacrylamide gel electrophoresis, and its electrophoretic mobility was identical to human serum albumin. This active fraction was significantly neutralized by rabbit antiserum to human serum albumin. These results suggest that the insulin-like activity in urine of obese subjects is more closely associated with albumin than with unmodified insulin.
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