Toxicity of L-Asparaginase to Resistant and Susceptible Lymphoma Cells in vitro ∗

Abstract

Summary and conclusions

The 6C3HED lymphoma which is susceptible to E. coli L-Ase in vivo, is also susceptible to E. coli L-Ase in vitro. The 6C3HED-ECLAR1 lymphoma which is resistant to E. coli L-Ase in vivo is also resistant to E. coli L-Ase in vitro. At 2 days of incubation, these cells respond to L-Ase concentrations of 1,000 to 10,000 fold of that needed to exert a cytotoxic effect upon the susceptible cell line. Spleen cells are approximately as resistant as the 6C3HED-ECLAR1 lymphoma. Thymus cells are more susceptible than spleen cells, but more resistant than the susceptible tumor cell line.