Abstract
Summary
A rapid simple method of obtaining large amounts of antibody gamma globulin from horse anti-lymphocytic serum (HALS) is described. Undialyzed serum was used and DEAE-Sephadex was employed as the anionic exchanger using a “batch” method. Cytotoxic antibody titers, pH and serum volume were unchanged during absorption of erythrocyte antibodies and purification. The final preparation was electrophoretically pure, gave a single 6.76 peak on the ultracentrifuge, and was structurally unaltered as evidenced by in vivo traces studies, and immunoelectro-phoretic analysis. In vivo toxicity did not occur during long term intravenous administration of the purified preparation. The resultant lymphopenia was equal to that achieved with whole serum containing two times the gamma globulin and 7 times the total proteins.
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