Abstract
Summary
Molecular aggregates of bovine ribonuclease (BRN) and bovine gamma globulin (BGG) were formed by surface adsorption to monodisperse polystyrene latex particles of uniform size: 0.09, 0.29 and 0.8 μ. Homogenous suspension of 7.6 × 106 untreated particles (P) and corresponding size BRN or BGG coated particles (CP) were given into opposite hind foot pads of guinea pigs. Eosinophil granular leukocyte infiltrations were demonstrated within microscopic sections prepared from regional popliteal lymph nodes removed at 6 and at 24 hour intervals after P or CP injections. Stained CP could be identified within the cytoplasm of eosinophils suggesting the occurrence of phagocytosis. Quantitated cell responses to P and CP in groups of 6 identically treated animals did not show significant differences for particle size within the 0.09-0.8 μ range, surface character, or specificity of the protein carried. It is suggested that the eosino-tactic effects of proteins in non-sensitized animals may be a function of their physico-chemical character as represented by the molecular aggregated state.
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