Abstract
Summary
1. Assays for N- and ring-hydroxylation of 2-acetylaminorluorene (AAF) by hepatic microsomes from normal and 3-methylchloranthrene (MC) -treated rats, hamsters, mice, rabbits, and guinea pigs showed marked differences in these enzyme activities in normal animals of the different species and in the effects of MC-treatment. The microsomes of all species, except the guinea pig in which A′-hydroxylation could not be detected, showed increases in N-hy-droxylation on MC-treatment; the largest increase was observed with hamster liver microsomes. Only rat and mouse liver microsomes showed large increases in ring-hydroxylation activity on MC-treatment. Increases in N-hy-droxylation activity of hamster liver microsomes and in ring-hydroxylation activity by rat liver microsomes after MC treatment were inhibited by administration of actinomycin D or puromycin. 2. MC-treatment of rats or hamsters did not affect the reduction of N-hy-droxy-AAF by liver homogenates. 3. MC administration caused a decrease in urinary excretion of 7V-hydroxy-AAF by rats and an increased urinary excretion by hamsters.
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