Abstract
Summary
Rabbit spleen cells producing antibodies against mouse immunoglobulin antigens were detected by a modification of the hemolytic plaque technique. The peak cellular and serologic response occurred 7 to 8 days following primary immunization and had subsided completely by the fifteenth day. The absence of spontaneous background distinguished protein reactive PFC from those reactive with erythrocyte antigens.
We thank Mr. B. Harrell for expert technical assistance and Dr. M. Landy for useful suggestions in preparation of this manuscript.
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