Abstract
Summary
A sensitive method of assay for Mengovirus RNA has been investigated using an agar-L cells suspension plaque technique as previously described for poliovirus RNA-HeLa cells system. The activity of polybasic substances (DEAE-dextran and Poly-L-Ornithine) at different concentrations also has been studied. Both compounds cause an increase of infectious titer of Mengovirus RNA at a rate depending on their respective concentrations. When used at the optimal concentrations, DEAE-D shows enhancing activity of about one thousand times and PO of one hundred times with respect to the control conditions. When the drugs were used in combination, no synergistic effect was evident. The highest infectious titer (107.2 PFU per ml) was obtained using DEAE-D at a concentration of 800-1600 μg per ml as diluent fluid for the infectious RNA preparations.
The authors would like to acknowledge the valuable suggestions and help by Dr. Gebhard Koch and Dr. Hilton B. Levy.
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