Abstract
Summary
Rubella hemagglutinin of high titer was prepared by alkaline extraction of infected BHK-21/13S cells grown in suspension culture. After this treatment, soni-cation or tween-ether extraction increased hemagglutinin titer 4-16-fold. Optimal conditions for hemagglutinin titration using eryth-rocytes from 1- to 3-day-old chicks included a low temperature and a pH of 6.2. A limited number of stability tests indicated that alkaline extracted hemagglutinin was stable at — 70°C and +4°C. The hemagglu-tinin was used to detect rises in HI antibody in paired serum specimens. Hemagglutination inhibition antibodies were present in titers of 1:40′ to 1:320, 2 to 3 years after rubella infection.
The supervision of all tissue culture preparation by Mrs. Anna Hall and the excellent technical assistance of Miss Marianne Davis and Miss Ludie Holland are gratefully acknowledged.
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