Abstract
Summary
Satisfactory monolayer cultures were produced after freezing bovine embryonic kidney (BEK) and chicken embryo fibroblast cells in the presence of 7.5% DMSO in the storage medium and storage for varying lengths of time in liquid nitrogen vapor. The sensitivity of such cultures to selected viruses remained undiminished after 6 months of such storage. BEK cells frozen after initial growth as primary monolayer cultures generally provided satisfactory mono-layer cultures within a relatively short time (2 to 4 days) as compared with BEK cells which were frozen after fresh procurement from tissues (5 to 8 days).
The authors wish to acknowledge the excellent technical assistance of Mr. Horace C. Turner and Mr. Monty Bell.
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