Detection of Five Components Having Antibody Activity in Rat Antisera. ∗ †

Abstract

Summary

By radioimmunoelectrophoresis, antibodies associated with γ₁, γ₂ and γM globulins and 2 unidentified components, U₁ and U₂, were detected in rat antisera to BSA and BGG. When rats were immunized with these antigens adsorbed to bentonite or included in complete Freund's adjuvant, the sequence of the immune response in rats involves first the formation of γ₁ antibody followed upon further immunization by γ₂ antibody. Generally, γM and the unknown components, U₁ and U₂, only appeared in hyperimmune sera.