Abstract
Summary
Methods have been described for the preservation of sheep erythrocytes by brief treatment with glutaraldehyde. These cells could be conjugated to proteins in the presence or absence of coupling reagents and were specifically agglutinated by specific anti-protein sera. These antigen-conjugated cells were stable for at least 6 months. Utilizing these cells, the inhibition of hemagglutination was found to be a sensitive technique for detection of small amounts of antigen.
The authors appreciate the excellent technical assistance of Mr. Weldon W. Harold. They are grateful to Dr. John R. Murphy, Department of Medicine, Western Reserve University School of Medicine, for informing them that erythrocytes could be fixed by brief exposure to glutiaraldehyde.
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