Abstract
Summary
The influence of corticosteroids on the incorporation of L-leucine-C14 into protein by ribonucleoprotein (RNP) particles from rat liver was studied. When RNP-particles from livers of adrenalectomized animals are used, the C14 amino acid incorporated is more than twice that with RNP-particles from normal rats. The increased incorporation is eliminated by administration of corticosterone to the adrenalectomized animals but not by direct addition of this hormone to the incubation system. In the incubation system used, RNP-particles from livers of normal and adrenalectomized rats incorporated C14-labeled amino acid at a rate proportional to the time of incubation. Preliminary electrophoretic separation of the labeled protein resulted in two protein peaks, the first representing 14% and the other 86% of the total protein, with the radioactivity associated entirely with the first protein peak.
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