Prevention of SV 40 Virus Tumorigenesis by Irradiated,Disrupted and Iododeoxyuridine Treated Tumor Cell Antigens. ∗

Abstract

Summary

Hamster SV₄₀ tumor cells rendered nonproliferative by exposure to gamma rays or by propagation in the presence of iododeoxyuridine were highly effective when used as immunizing antigens for preventing the appearance of tumors in hamsters which had received SV₄₀ virus when newborn. Only a single injection of immunizing antigen was employed. Disruption of tumor cell preparations by freeze-thaw or by treatment in a French pressure cell for purpose of fractionation resulted in total or near total loss of immunizing capability, even when incorporated into alum adjuvant. Possible mechanisms for loss of potency are discussed.

Addendum: Since the time the present manuscript was in press, it was found in further experiments employing the same techniques that the loss of RNA and protein following cell disruption was considerably less than noted above.