Abstract
Summary
By means of a blind test, in which serum samples from a group of rabbits were relabeled with code designations, it was shown clearly that definite antibody production had occurred as a result of freezing of tissue in the animal. Freezing was done by means of a liquid nitrogen probe and the target tissue was the coagulating gland and seminal vesicle of the urogenital tract. Antibodies were detected by tanned cell hemag-glutination and were found to be present within 7 days after cryosurgery. No antibody was detected in any of 15 sera from 5 control animals, whereas 6 out of 7 rabbits exposed to freezing showed positive results. Titers as high as 1:4096 were obtained, and there was a progressive increase with time.
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