Abstract
Summary
Specific heterophile antibody was prepared by adsorption to beef erythro-cytes and elution with diethyl ether. Using a formula based on the antigen-antibody relationship permitting maximum elution, up to 10% of the antibody was recoverable. The eluted material, further purified by sucrose density gradient ultracentrifugation, had the characteristics of heterophile antibody. The material was found to be unstable at —20°C or 4°C storage, but was relatively stable in 30% sucrose and 50% glycerin at 4°C.
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