Abstract
Summary
Administration of the carcinogen 2-acetylaminofluorene (AAF) orally or by injection into rainbow trout resulted in the excretion of 5- and 7-hydroxy-AAF in the urine and feces. These metabolites were also formed in vitro from AAF by trout liver homogenates or the microsomal fraction in the presence of TPNH or a TPNH-generating system. TV-Hydroxylation of AAF was not demonstrated either in vivo or in vitro.
We are grateful to Mrs. Helen P. Newton for excellent technical assistance, and to Mr. Robert R. Smith, Manager, Western Fish Nutrition Laboratory Field Station, Hagerman, Idaho, for his conscientious and meticulous care of the cannulated trout.
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