Abstract
Summary
Single cell clones were derived from 4 lines of human leukemia and Burkitt lymphoma cells in long-term culture by a semi-solid agar procedure. Some clonally derived cell strains were shown to produce only one component of heavy chain immunoglobulins and others produced 2 components as determined by immunofluorescence. One of 4 cell lines tested contained 2 different types of cells. One cell type produced only Ig M. The other cell type produced both Ig M and Ig G. All of the clones derived from 3 other cell lines produced the same immunoglobulins as their parent cultures.
We thank Mr. Donald Wudarski for excellent technical assistance and Dr. M. Konn for assistance with the immunofluorescent studies.
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