Abstract
Summary
Two volunteers were given adenovirus type 27 by conjunctiva! inoculation, followed in 3 months by a rechallenge with type 27, and a month later by a heterotypic cross-challenge with type 26. Sequential serum specimens were fractionated by density gradient ultracentrifugation or by gel filtration; pools containing a preponderance of IgM, IgA or IgG were prepared from each specimen; and homotypic and heterotypic HI and neutralization antibody titers were measured in each serum specimen and immuno-globulin pool.
No 19S IgM homotypic or heterotypic antibody activity was detected.
Titers of IgG and IgA antibody rose sharply 2 weeks after the initial inoculation, and the pattern of their appearance was much the same as the pattern of appearance of antibody in unfractionated serum. No significant boosts of antibody titers were demonstrable after rechallenge or cross-challenge. Antibody remained present in serum and in “IgG” and “IgA” pools for the 18-week duration of the study.
Get full access to this article
View all access options for this article.