Abstract
Summary
By means of gel nitration on Sephadex G-200, rubella complement-nxing antigens of two distinct particle sizes have been demonstrated in concentrates of fluids from infected BHK-21 cultures. The large-particle antigen elutes from Sephadex columns together with infectious virus, shortly after the void volume is eluted, and is sedimented by centrifugation at 80,000 × g for 3 hours together with the infectious virus. The small-particle antigen elutes after the infectious virus and is not sedimented under conditions of centrifugation which sediment the infectious virus. Sephadex gel nitration was shown to be more effective than direct centrifugation of rubella-infected fluids in separating the two different antigens.
Get full access to this article
View all access options for this article.