Abstract
Summary
An agar gel electrophoretic method has been described for separation of both nonradioactive and tritiated thymine, thymidine and thymidine-5′-monophosphate. The results obtained by this technique were faster and simpler, but comparable to those of column chromatography of bone marrow fluids. The radioactive samples separated on a microscope coverglass were subsequently scanned using an automatic gas flow counter. This quantitative procedure was useful for the study of nucleosides and nucleotides separated by agar gel electrophoresis.
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