Amino Acid Utilization by Isolated Adipose Tissue of Meal-Fed Rats

Abstract

Summary

Meal-feeding (limiting access to food to 2 hours per day) in the rat stimulated the utilization of leucine- and lysine-¹⁴C by isolated adipose tissue. Epididymal adipose tissue from meal-fed rats incorporated significantly more amino acid carbon into protein and fatty acids than did similar tissue from nibbling (ad libitum-ied) animals. Greater quantities of amino acids were also oxidized by adipose tissue of meal-fed rats. The incorporation of leucine- and lysine-¹⁴C into protein and fatty acids was stimulated by addition of glucose to the incubation medium, and insulin addition resulted in a further stimulation. The possible regulatory mechanisms involved are discussed in light of available knowledge concerning adipose tissue metabolism.