Abstract
Summary
Corpora lutea of isolated luteal-phase swine ovaries perfused with a blood substitute synthesize progesterone without being stimulated by HCG. Synthesized progesterone was isolated by solvent extraction and thin layer chromatography. The isolated progesterone was identified by thin layer chromatography, gas chromatography, ultraviolet absorption spectra, and 2, 4 dinitro-phenylhydrazone derivatives. Small but detectable amounts (less than 0.5% of the amount theoretically possible) of acetate-1-C14 were incorporated into the synthesized progesterone. Most of the progesterone (88% ± 12%) was found in the corpora lutea with the remainder in the perfusate. An average value of 43 μg of progesterone per gram of luteal tissue was synthesized during the per fusion period. Newly synthesized progesterone represented an average value of 45% of the total progesterone isolated from corpora lutea and perfusate.
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