Uptake of Dihydrostreptomycin by Intact Splenic Cells Cultured from Immunized Rabbits and its Binding to the 40S Ribosomal Subunit. ∗

Abstract

Summary

Tritiated dihydrostreptomycin (H³-DHSM) was taken up by intact splenic cells cultured from rabbits immunized with bacteriophage MS-2 and was bound to the 40S ribosomal subunit of the 76S splenic ribosome. Splenic cells that had been maintained in the presence of 200 /xg/ml unlabeled DHSM took up as much H³-DHSM as cells not previously exposed to the antibiotic. However, only cells that had not been cultured in the presence of DHSM bound H³-DHSM in cold trichloracetic acid-insoluble form. The binding of H³-DHSM by splenic cells was reversible and dependent on the concentration of Mg++ in the suspending medium.