Demonstration of Tissue Receptors for Viruses.

Abstract

Summary

By using a principle analogous to that of mixed agglutination, virus-treated RBC may be caused to adhere to tissue sections in a slide chamber test. The adherence depends on the presence of virus receptor sites on both tissue and RBC; thus the occurrence of hemadsorption serves as a useful indicator for the presence of receptor sites in the tissue tested. Treatment of tissue or RBC with RDE prevents the occurrence of hemadsorption, as does treatment of the tissue with periodate. In the chick embryo, infection with NDV seemed to eliminate tissue receptors for this virus. The technique should prove applicable to studies of species susceptibility to viral infection and to studies of organotropism of viruses.