Studies on Induction of Virus from Adenovirus and SV 40 Hamster Tumors 1. Chemical and Physical Agents. ∗

Abstract

Summary

Cell culture lines of virus-free hamster tumors initiated by SV₄₀ virus or by the Pinckney and Grider strains of adenovirus 7 were tested exhaustively by procedures which might be hoped to induce the virus or to increase its chance for detection. This included treatment with actinomycin D, mito-mycin C, puromycin, proflavine, hydrocorti-sone, ACTH, thyroxine, insulin, H₂O₂, increased O₂, γ-radiation and copropagation with indicator cells. In no case was infectious virus recovered. Superinfection of SV₄₀ hamster tumor with SV₄₀ virus could not be established and hypothetical inapparent super-infection was not demonstrated following treatment with mitomycin C, proflavine or H₂O₂. No virus was detected in the cell cultures of any of 3 human tumors tested. The findings supported the concept that viral genetic materials in the animal tumors which are sufficient to code for “T” antigen may be insufficient to replicate the complete infectious virus.