Incorporation of Protein and Nucleic Acid Precursors into Frog Nervous Tissue in vitro,

Abstract

Summary

The effects of TCAP on the incorporation of C¹⁴-adenine, C¹⁴-guanine, H³-uridine, H³-cytidine, and C¹⁴-valine into frog central and peripheral nervous tissue maintained in vitro was observed. TCAP inhibited C¹⁴-valine incorporation into the protein fraction of both tissues. However, H³-uridine incorporation into RNA was inhibited only in brain tissue. TCA.P caused changes in the nucleic acid metabolism of frog ganglia in general quite similar to those produced by DNP and azide. However, TCAP did inhibit adenine incorporation into RNA whereas DNP and azide did not.