Abstract
Summary
Blood from the donor was drawn into 5 successive bottles, each containing varying amounts of citrate, heparin, glucose, phosphate, adenine, guanosine, and ino-sine. The blood was stored under blood bank conditions for up to 6 weeks and samples were aseptically withdrawn at intervals for column chromatographic assay of ATP and nucleo-tide adenine (AMP + ADP + ATP). Phosphate was important in maintaining ATP and nucleotide adenine but at least 5 mM (final concentration in collected blood) was required. Adenine (0.5 mM) was as effective as phosphate, and the effect of the two was additive. Heparin, especially in the presence of phosphate, was not markedly superior to citrate in its effect on red cell metabolism. Citrate could be used at half the concentration in ACD without apparent clotting. Guanosine (0.5 mM) was definitely beneficial at 6 weeks, although without much apparent effect at 3 weeks. Inosine did not improve results in 4 weeks and was not tested at 6 weeks.
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