Insulin Antibodies

Abstract

Summary

1. The gel filtration technique was used to separate free from bound insulin in sera and hence to indicate the presence of insulin binding antibodies in the circulation of insulin-treated diabetic individuals. 2. Although most of the sera showed the presence of only one γ-globulin insulin complex, 2 such complexes were observed in 11 of the 35 treated diabetics whose sera was tested for the presence of anti-insulin antibodies. 3. The formation of Ag-Ab and Ag-Ab₂ in some patients (9) is postulated to explain the presence of 2 protein bound radioactivity peaks in the inter AB and B protein peaks, respectively. 4. Analytical ultracentrifugation was used to verify the presence of both 19S and 7S anti-insulin antibodies in 2 cases.