Abstract
Summary
Evidence was presented which indicates that HGH activity obtained by an alkaline extract of pituitaries is distributed in at least 3 different molecules or aggregates which varied widely in respect to size. Ribonucleic acid was also shown to be present in these fractions. The nucleic acid could not be removed from the GH by chromatog-raphy, electropartition or isoelectric precipitation. It could be removed by digestion with ribonuclease. Upon digestion by ribonuclease the activity was located in molecules or aggregates more homogeneous with respect to size, but with different isoelectric points. A further digestion by phosphodiesterase resulted in the activity being located in a fraction more homogeneous with respect to isoelectric point. It was suggested that the majority of the HGH in the pituitary is bound to ribonucleic acid as a macromolecule.
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