Metabolism of 1-131 by Human Thyroid Tissue in Organ Culture. ∗

Abstract

Summary

The incubation of human thyroid tissue in tissue culture medium containsing I-131 by an organ culture technique is described. The histology of the gland was preserved up to 7 days. Labeled MIT, DIT, and T⁴ were present following papain hydrolysis of the tissue. Addition of TSH to the culture medium apparently accelerated the conversion of MIT to DIT as well as the rate of coupling of DIT to form T⁴ and released T⁴ from the thyroid tissue into the medium. No labeled amino acids were detected in the culture medium when TSH was not added.