Carcinogenic Effect of N-Hydroxy-N-2-Fluorenylacetamide,2′,4′-Dimethylacetanilide,and 2′,4′,6′-Trimethylacetanilide on Liver in Suckling Mice

Abstract

Summary

Suckling C57BL₆/A_JF¹ hybrid mice were fed the following compounds suspended in methocel-Aerosol O.T. by stomach tube beginning at 1 week of age and continuing for approximately 3 weeks: N-hydroxy-N-2-nuorenylacetamide (N-OH-FAA), 2′4′-dimethylacetanilide, and 2′4′6′-trimethylacet-anilide. Acetanilide and the vehicle were similarly administered as controls. Approximately 50% of the male mice, but none of the females, developed hepatomas in groups fed a total of 2.5 mg over several weeks, or 0.5 mg of N-OH-FAA in one day, average number of tumors per liver being greater in the higher dosage group. An occasional hepatoma was observed among males exposed to the other 2 test compounds while none appeared in either of the control groups. The data demonstrate that a. minute amount of N-OH-FAA had a. potent tumorigenic effect in the liver when tested in infant male mice.