Attempts to Find Creatine Phosphokinase and 5′-Nucleotidase Activity In Canine Prostatic Fluid. ∗

Abstract

Summary

An attempt was made to establish the presence of creatine phosphokinase and 5′-nucleotidase activities in canine prostatic secretion. Pooled prostatic fluid and purified enzyme fractions were utilized as sources of enzyme. Transferase activity was followed by phosphorylation of creatine and dephosphorylation of phosphocreatine, and 5′-nucleotidase activity by hydrolysis of ade-nosine-5′-phosphate. No creatine phosphokinase activity could be detected in the sources of enzyme but a small amount of 5′-nucleo-tidase activity may have been observed. The latter could not be distinguished from prostatic acid phosphatase by purification procedures or inhibition with tartrate and sodium fluoride.