Abstract
Summary
Gas-liquid radiochromatography has been successfully applied to the simultaneous analysis of mass and radioactivity of labeled intact cholesterol esters. Representative curves are presented for labeled cholesterol esters isolated from rat serum, liver, and adrenal gland. Quantitative mass and radioactive measurements were carried out on the labeled cholesterol esters isolated from the liver of rats given cholesterol-4-C14 and cholesterol- 7α-H3. The results indicate that gas-liquid radiochromatography coupled with thin-layer chromatography affords an excellent means for determining the specific radioactivity of labeled cholesterol ester classes.
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