Abstract
Summary
Mycoplasma gallisepticum strain J has been grown for over 100 serial subcultures in a medium in which the only non-defined components were Trypticase, Hycase and TEM 4T. Hycase can be replaced by an amino acid mixture, with a slightly lower plane of growth. An interchangeable requirement for pantethine, pantetheine or coen-zyme A was demonstrated. Calcium pan-tothenate had very low activity and panthe-nol was inactive. Biotin and vitamin B12 stimulated acid production during growth.
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