Abstract
Discussion and Conclusions
Significant changes in lactic dehydrogenase isoenzyme patterns of plasma and of extracts of several organs were seen in rats which had been shocked in the Noble-Collip drum. The changes were observed 3 hours after drumming. Increase of lactic dehydrogenase in the plasma of dogs and of rabbits in hemor-rhagic shock has been described by Vessell, Feldman and Frank(4), who have also carried out electrophoresis of lactic dehydrogenase in dog plasma and have observed 4 fractions. No difference in the relative activity of each fraction was seen in shock: all fractions were elevated to approximately the same degree.
We find that after administering tumbling shock to rats the isoenzymes of LDH measured in extracts from different organs are differently affected. In agreement with numerous other observations the plasma enzyme is elevated throughout suggesting damage to several organs and leakage of enzymes into the blood stream. Individual organs, however, do not show uniform responses. In the kidney the response to both challenge and recovery are most outspoken: 3 of 5 isoenzymes show significant increases and one significant decrease, in shock. In the OHP treated shocked rat all differences of kidney isoenzymes from the normal animal disappear. Two of the isoenzymes from shock heart preparations show return to the normal range after OHP treatment. No consistent effects were noted by us in the brain in shock, with and without subsequent OHP treatment.
We acknowledge gratefully the assistance of Doctor E. H. LaBrosse in the editing, and the cooperation of Doctor M. L. Tayback and Mr. E. Jenkins in the statistical analysis.
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