Abstract
Summary
Sodium deoxycholate (SDC) in various concentrations markedly affected the biological activities of measles virus. Infectivity was destroyed at concentrations of 0.01 to 0.05%. The HA activity was enhanced by SDC in concentrations of 0.01 % or higher. The maximum increase, 10- to 50-fold, was obtained with 0.05% SDC, whereas the yield at still higher concentrations was progressively lower. The major part of HA released by SDC treatment of intact and almost intact virions had a sedimentation coefficient of 13 to 16S. A method was worked out for separation of hemagglutinating and non-hemagglutinating CF antigen by use of repeated differential centrifugations in combination with SDC treatment.
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