Abstract
Summary
A simplified technique for studying the uptake of radio-isotope labelled precursors in normal and neoplastic cells in tissue culture is described. It makes use of the fact that uniform discs can be cut from the cell-covered bottom of plastic tissue culture dishes with a modified cutting tool. The discs may then be counted directly in an automatic gas flow isotope counter or mounted on glass slides and coated with photographic emulsion for high resolution autoradiography or stained by a variety of cytological procedures. Rapidity and simplicity of handling as well as reproducibility of samples are distinct advantages.
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