Effect of Marginal Intakes of Pyridoxine on Acetate-2-C 14 Incorporation into Rat Liver Lipids. ∗

Abstract

Summary

The purpose of the present study was to determine whether marginal intakes of pyridoxine would produce the increased incorporation of acetate-C¹⁴ into liver sterol previously observed in rats severely deficient in pyridoxine. Male weanling rats were fed 6, 12, or 36 μg of pyridoxine hydrochloride daily for 9 weeks, then injected introperitoneally with sodium acetate-2-C¹⁴, and incorporation of C¹⁴ into liver sterol determined. C¹⁴ incorporation (cpm/mg) by the group fed 6 μg was greater than the incorporation by the group fed 12 or 36 μg. C¹⁴ incorporation (cpm/mg) was the same, however, in the groups fed 12 or 36 μg pyridoxine hydrochloride even though the growth of the former group was significantly less. It was concluded (1) that quite a severe deficiency of pyridoxine was required to produce the conditions resulting in an increased incorporation of acetate-C¹⁴ into liver sterol and (2) that this effect, which might be interpreted as increased sterol synthesis, did not occur in moderate pyridoxine deficiency, under the conditions of this experiment.