Abstract
Summary
Methods have been described for separating the γ1- and γ2-globulin antibodies and the macroglobulin antibody of guinea pig sera. Use of simple stepwise elution chromatography on DEAE-cellulose results in extensive purification of γ2-globulin antibody. Purified macroglobulin antibody was prepared from relatively large amounts (170 ml) of appropriate sera by combined use of salting out, ion-exchange chromatography and gel filtration.
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