Abstract
Summary
Trichloroacetic acid (TCA) extracts of moldy hay contained 3 antigens which produced precipitins with a typical farmer' lung serum. These antigens were fractionally precipitated with ethanol; antigens were present in the fractions precipitated between the limits of 50 to 60%, 60 to 70%, and 70 to 90% alcohol. Each of these alcohol fractions was further purified on diethylaminoethyl (DEAE-) cellulose and analyzed for sugars and amino acids. It is concluded that these TCA-soluble antigens involved in farmer' lung disease are composed of peptides and carbohydrates which were not separated by TCA extraction, alcohol fractionation, or by chromatography on DEAE-cellulose ion exchange columns.
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