Abstract
Summary
The sucrose polymer Ficoll at the proper concentration activates the proteinaceous growth factor necessary for continuous cultivation of the free-living nematode, C. briggsae, in a chemically denned medium. It increases the activity that is produced by other activating procedures. It increases uniformity of maturation, allows greater stability at 37°C, and produces a mixture with growth factor that can be readily lyophilized. Its use thus greatly extends the usefulness of the growth factor.
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