Abstract
Summary
The replication of simian virus 5 in rhesus monkey kidney cells was not inhibited by 5-fluoro-2′-deoxyuridine, 5-bromo-2′-deoxyuridine, or 5-iodo-2′-deoxyuridine. Actinomycin D did not inhibit viral replication during the first 10 hours of viral growth, and the addition of the drug to cells which were already producing virus did not inhibit continuing virus production until after 10 hours. However, concentrations of actinomycin as low as 0.1 μg/ml significantly reduced the virus yield obtained at 22 hours or later. The results suggest that neither DNA synthesis nor DNA-dependent RNA synthesis is required for the synthesis of the virus, and that the inhibition of the continued production of virus is secondary to effects on the host cell.
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