Abstract
Summary
Human blood and marrow leukocytes were studied both cytochemically and biochemically for alkaline phosphatase activity. Naturally occurring nucleotides, as well as synthetic compounds, were tested as substrates for leukocytes from varied patients. All substrates reacted similarly with granulocytes from normal subjects. Granulocytes from patients with selected diseases showed proportional changes in reactivity for all substrates. These results suggest the presence of one common alkaline phosphatase. Dephosphorylation of thymidylate was consistently demonstrable. This unique deoxynucleotide may be an important natural substrate for granulocyte alkaline phosphatase.
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