Abstract
Summary
Purification of the TRF concentrate prepared by gel filtration of acetic acid extracts of hypothalamic tissues on Sephadex G-25 can be achieved following gel chromatography on carboxymethyl-Sephadex C-50 equilibrated in 0.001M pyridine acetate. The TRF activity is quantitatively eluted upon stepwise application of 0.09M pyridine acetate buffer. The method described can be used with small columns (4 × 15 cm) with charges ≤ 1 g TRF concentrate and also with large size preparative columns (15 × 33 cm) with charges up to 25 g of the TRF concentrate.
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